News beyond our pages – July 2025

Translational research involving adoptive switch of tolerogenic regulatory T (Treg) cells have been hindered by phenotypic instability of in vitro-induced Treg (iTreg) cells. Chen et al (Nature 2025 Mar 26; https://doi.org/10.1038/s41586-025-08795-5) carried out an unbiased genome-wide display screen utilizing clustered commonly interspaced quick palindromic repeats (CRISPR)/Cas9 expertise in main human T cells to determine novel regulators of FOXP3 expression. Candidates have been validated by integrating transcriptomic profiling and intracellular protein abundance at a single-cell degree.

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