A Guide to Single-Cell Sequencing

Single-cell sequencing is a helpful device for analyzing advanced mobile populations, enabling high-resolution views of the transcriptome, epigenome, and immune repertoire that aren’t attainable by means of typical bulk sequencing. This eBook explores the basics of single-cell sequencing, specializing in how this expertise works, optimized approaches to boost outcomes, and use-case purposes that can assist you get essentially the most out of your single-cell analysis.

What You will Study:

• The fundamentals of single-cell sequencing, together with the commonest sorts of single-cell strategies and key concerns for mission design
• Leveraging lifeless cell removing to beat low cell viability and generate high-quality sequencing outcomes
• Alternate cell fixation strategies for getting ready single-cell samples, with out sacrificing integrity or knowledge high quality
• Utilizing single-cell evaluation to determine uncommon cells, characterize their transcriptomes, and uncover potential biomarkers
• Excessive-resolution spatial profiling and biomarker discovery utilizing spatial transcriptomics expertise