For our move cytometry peeps, would you prefer to have a single repair/perm protocol that’s optimised for every part? One which preserves fluorophores whereas permitting simultaneous TF and cytokine staining? How a few protocol that’s 100-fold cheaper than your present one?
During the last 8 years, Oliver Burton has examined >1000 totally different repair/perm combos, and right here the ultimate verdict is: “Burton’s Greatest Buffer”: 2% formalin, 0.05% Fairy dish cleaning soap, 0.5% Tween-20, 0.1% Triton X-100.
Yep, substitute all of these costly detergents with Fairy dishwashing liquid. It’s pretty much as good because the BD Foxp3 repair/perm equipment for transcription elements, pretty much as good as eBio perm for cytokines, preserves even weak endogenous GFP killed by most repair/perm combos, and preserves dye integrity too. Burton’s Greatest Buffer is solely the very best repair/perm protocol to make use of below any situation (besides phospho-flow).
Plus it’s filth low-cost – one bottle of Fairy (or Dreft, Daybreak, Sure, JAR, or no matter they promote it as regionally) will actually final your lab for many years.
Take a learn of the protocol here.
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