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MRGPRX2 facilitates IgE-mediated systemic anaphylaxis in a newly established knock-in mouse model

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Along with FcεRI, a subtype of human mast cells (MCs) expresses Mas-related G-protein coupled receptor X2 (MRGPRX2, mouse counterpart MrgprB2). Though MrgprB2 contributes to IgE-mediated passive systemic anaphylaxis (PSA) in vivo, an MRGPRX2 inhibitor (C9) doesn’t block MrgprB2-mediated or IgE-mediated MC degranulation in vitro. Goal: To generate mice expressing human MRGPRX2 to review receptor perform in vitro and PSA in vivo.

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