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Peptide Barcodes for Next-Generation Protein Sequencing™

Barcoding applied sciences that benefit from the excessive data content material that may be encoded in oligonucleotide sequences have enabled a wide selection of purposes in biotechnology, significantly when coupled with next-generation DNA sequencing (NGS) to decode this data in a high-throughput, cost-effective method. For instance, DNA barcoding mixed with NGS read-out is employed to trace pattern identification in multiplexed libraries, present single-cell or spatial decision in transcriptomic research, and to trace the enrichment of genotypes in directed evolution strategies for protein engineering.1–3 Peptide barcodes, with their capability to encode intensive data in brief sequences, simple genetic encoding, and chemical versatility, current cutting-edge alternatives. Functions of peptide barcoding that use mass spectrometry for decoding, similar to nanobody screening based mostly on flycodes,4 have been developed. Nonetheless, there stays a crucial want for accessible strategies to straight learn peptide barcode sequences and to determine peptide barcodes with single-molecule decision. Subsequent-generation protein sequencing™ (NGPS) on Quantum-Si’s Platinum® instrument presents researchers the flexibility to straight sequence peptide barcodes with single-molecule decision for the primary time. This user-friendly benchtop platform combines the ease-of-access of DNA-based strategies with the thrilling, revolutionary capabilities of peptide-based barcoding approaches.

Discover Quantum-Si’s application note for standards on peptide barcode design, library technology strategies, and sensible examples of how peptide barcodes revolutionize protein screening, together with a 300-fold enrichment in nanobody abundance after optimistic choice utilizing peptide barcodes.

 

References:

  1. Wang, Y. & Navin, N. E. Advances and Functions of Single-Cell Sequencing Applied sciences. Mol. Cell 58, 598–609 (2015).
  2. Whitmann, B. J., Johnston, K. E., Almhjell, P. J. & Arnold, F. H. evSeq: Price-Efficient Amplicon Sequenc- ing of Each Variant in a Protein Li- brary. ACS Synth Biol 11, 1313–1324 (2022).
  3. Baysoy, A., Bai, Z., Satija, R. & Fan, R. The technological panorama and purposes of single-cell multi-omics. Nat. Rev. Mol. Cell Biol. 24, 695–713 (2023).
  4. Egloff, P. et al. Engineered Peptide Barcodes for In-Depth Analyses of Binding Protein Ensembles. Nat. Strategies 16, 421–428 (2019).

 

 

 

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